Gotu Kola extract Asiaticoside 10%-90%

GUTO kola

Product Name: Gotu Kola extract Asiaticoside 10%-90%

Botanical Name: Centella Asiatica

Part Used: Whole plant

Active Ingredient: Asiaticoside 10%-90%

CAS No.: 16830-15-2

Molecular Formula: C48H78O19

Specification: 10%, 20%, 30%,80%,90% Asiaticoside

Appearance: Lightless yellow to white powder

Main benefits of  Asiaticoside 10%-90%: 

1.Anti-inflammatory:
Anti-irritant.
2.Dermis reconstruction:
Enhance the collagen synthesis.
3.Epidermal reconstruction:
Improvement of the keratinization functionality.
4.Anti-aging:
Dermal cells stimulation.

Application of  Asiaticoside:

Asiaticoside is capable of stimulation of collagen and glycosaminoglycan synthesis.It is also found to have activity of angiogenesis and against herpes simples virus.Asiaticoside is commonly used in wound healing,endangium restoring, connective tissue structure enhancing.

 

HPLC to analysis Asiaticosides in  Gotu Kola Extract

Instrument

Water 515, UV-2487

HLPC mobile phass

Acetonitrile:Methanol:Water(26:24:50)

Sample preparation

Weight 25mg extract exactly into 25ml dissolution flask.

Add 8ml HPLC grade methanol and ultrasonic bath until sample has dissolved, Cool to room temperature , then diluting to volume with HPLC grade methanol.

Filtering sample through a 0.45µm membrane filter .

Preparation of standards solution

Each weight dried asiaticoside and madecassoside  to make the standards solution with the concentration of 0.8mg/ml (Cs).

HPLC chromatogram condition

Column: Hypersil C18, 4.6mm I.D×200mm, 5µm

Flow rate : 0.8ml/min

Column temperature: 35℃

Inject volume:5µl

Detect Wavelength: UV 205nm

 

Asiaticoside  90%

Standard Specification
Test item Standard
Appearance White to creamy powder
Loss on drying <3.0%
Assay Asiaticoside > 90%
Sulphated ash <1.0%
Pb / Hg / Cd / Cu / As <5 / <0.2 / <0.3 / <5 / <2 ppm
BHC / DDT / PCNB / Aldrin <0.1 / <0.1 / <0.1 / <0.02 ppm
Residual methanol <0.2%
Residual ethanol <0.002%
Heavy metals <10 ppm
Yeast/moulds Less than 100 cfu/g
Total bacteria count Less than 1000 cfu/g
Salmonella Negative
E-Coli Negative
Pseudomonas aeruginosa Negative
Staphylococcus aureus Negative

 Asiaticoside  10%-95%

Name Asiaticoside
Botanical Source Centella asiatica (L.) Urban
Maker Compounds Asiaticosides
Molecular Formula C48H78O19
Molecular Weight 959.12
Test Method HPLC
Color Brown-yellow to White
Part Used The whole herb
Appearance Fine Powder
Ash Content ≤5%
Extract solvent Ethanol & Water
Drying Method Spray Dying
Particle Size NLT100%Through 80 mesh
Loss on Drying ≤ 5.0%
Bulk Density 40-60g/100ml
Arsenic ≤2ppm
Lead ≤2ppm
Total Plate Count ≤1000cfu/g
Total Yeast & Mold ≤100cfu/g

 


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